Fluorous tagged small molecule microarrays.
نویسندگان
چکیده
The affinity fluorous interaction between fluorous tagged small molecules and a fluoroalkyl modified glass surface was shown to facilitate the detection of protein-ligand binding interactions in the fabrication and screening of small molecule microarrays.
منابع مشابه
Fluorous-based carbohydrate microarrays.
The success of microarrays, such as DNA chips, for biosample screening with minimal sample usage has led to a variety of technologies for assays on glass slides. Unfortunately, for small molecules, such as carbohydrates, these methods usually rely on covalent bond formation, which requires unique functional handles and multiple chemical steps. A new simpler concept in microarray formation is ba...
متن کاملSynthesis and Solid State Structure of Fluorous Probe Molecules for Fluorous Separation Applications.
A series of colored hydrocarbon and fluorocarbon tagged 1-fluoro-4-alkylamino-anthraquinones and 1,4-bis-alkylamino-anthraquinone probe molecules were synthesized from a (fluorinated) alkyl amine and 1,4-difluoroanthraquinone to aid in the development of fluorous separation applications. The anthraquinones displayed stacking of the anthraquinone tricycle and interdigitation of the (fluorinated)...
متن کاملSynthesis and quantitative evaluation of Glycero-D-manno-heptose binding to concanavalin A by fluorous-tag assistance.
tion was reported earlier in the first demonstration of a microarray fabrication strategy based on noncovalent fluorous interactions. Mannose and other monosaccharides tagged by single C8F17 chains were noncovalently immobilized to fluorocarbon-coated glass slide surfaces and shown to tolerate conditions necessary for identifying binding partners such as conA. More recently, this fluorous-based...
متن کاملFluorous methods for synthesis and separation of organic molecules*
Fluorous molecules partition out of an organic phase and into a fluorous (highly fluorinated) phase in a liquid–liquid extraction. New fluorous techniques allow simple yet substantive separations of organic reaction mixtures based on the presence or absence of a fluorous tag. Fluorous-tagged molecules can also be separated from nontagged molecules by solid phase extraction over fluorous reverse...
متن کاملPlate-to-plate fluorous solid-phase extraction for solution-phase parallel synthesis.
A commercially available Argonaut VacMaster-96 plate-to-plate solid-phase extraction (SPE) station equipped with 24 FluoroFlash cartridges is employed for parallel purification of fluorous reaction mixtures. Each cartridge charged with 3 g of fluorous silica gel has the capability to produce up to 100 mg of purified small molecules. The 24-well receiving plate has a standard footprint that can ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 38 شماره
صفحات -
تاریخ انتشار 2007